Additional file 2.

The schematic of pACYCDuet-lipA-lipBconstruction process. The constructed plasmids T-lipA which contained lipA gene was digested with BamHI and HindIII and ligated into similarly digested pACYCDuet-1 vector using T4 DNA ligase. The re-constructed plasmid pACYCDuet-lipA and the constructed plasmid T-lipB which contained lipB gene was digested with NdeI and XhoI, repectively. Then the two digested vectors were ligated using T4 DNA ligase to get the recombinant plasmid pACYCDuet-lipA-lipB.

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Wu et al. BMC Biotechnology 2012 12:58   doi:10.1186/1472-6750-12-58