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Open Access Research article

In vivo functional expression of a screened P. aeruginosa chaperone-dependent lipase in E. coli

Xiangping Wu, Pengyong You, Erzheng Su*, Jingjing Xu, Bei Gao and Dongzhi Wei*

Author Affiliations

State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China

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BMC Biotechnology 2012, 12:58  doi:10.1186/1472-6750-12-58

Published: 6 September 2012

Additional files

Additional file 1:

Genetic maps of recombinant plasmids pET28a-lipAB(A), pETDuet-lipA-lipB(B) and pACYCDuet-lipA-lipB(C).

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Additional file 2:

The schematic of pACYCDuet-lipA-lipBconstruction process. The constructed plasmids T-lipA which contained lipA gene was digested with BamHI and HindIII and ligated into similarly digested pACYCDuet-1 vector using T4 DNA ligase. The re-constructed plasmid pACYCDuet-lipA and the constructed plasmid T-lipB which contained lipB gene was digested with NdeI and XhoI, repectively. Then the two digested vectors were ligated using T4 DNA ligase to get the recombinant plasmid pACYCDuet-lipA-lipB.

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Open Data