Table 4

Effect of increasing cells on cell survival and transfection efficiency using the cell line L kit and T030 program
Program Cells pmaxGFP (μg) % Cell survival % Transfection efficiency % Cell input, alive and transfected
T-030 500,000 No GFP control 36.0 ±4.2* 0.4 ±0.2* 0.1
T-030 500,000 3 μg 19.0 ±0.8* 61.3 ±5.5* 11.6
T-030 1,000,000 3 μg 13.0 ±1.0# 88.5 ±2.3# 11.5
T-030 1,500,000 3 μg 11.0 ±1.2# 89.4 ±2.0# 9.8

Values ± SD with * represent the mean of 4 independent experiments, each with 4 biological replicates and values with # represent the mean of 2 independent experiments, each with 2 biological replicates.

Dwivedi et al.

Dwivedi et al. BMC Biotechnology 2012 12:45   doi:10.1186/1472-6750-12-45

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