Table 4 |
|||||
| Effect of increasing cells on cell survival and transfection efficiency using the cell line L kit and T030 program | |||||
| Program | Cells | pmaxGFP (μg) | % Cell survival | % Transfection efficiency | % Cell input, alive and transfected |
| T-030 | 500,000 | No GFP control | 36.0 ±4.2* | 0.4 ±0.2* | 0.1 |
| T-030 | 500,000 | 3 μg | 19.0 ±0.8* | 61.3 ±5.5* | 11.6 |
| T-030 | 1,000,000 | 3 μg | 13.0 ±1.0# | 88.5 ±2.3# | 11.5 |
| T-030 | 1,500,000 | 3 μg | 11.0 ±1.2# | 89.4 ±2.0# | 9.8 |
Values ± SD with * represent the mean of 4 independent experiments, each with 4 biological replicates and values with # represent the mean of 2 independent experiments, each with 2 biological replicates.
Dwivedi et al. BMC Biotechnology 2012 12:45 doi:10.1186/1472-6750-12-45
