Table 2

Effect of specific nucleofection programs on cell survival and transfection efficiency of human primary calvarial suture mesenchymal cells
V- Nucleofector buffer L- Nucleofector buffer
Nucleofection program pmaxGFP (μg) % Cell survival % Transfection efficiency % Cell survival % Transfection efficiency
No-Program control 3μg 99.2 0.2 98.8±0.7* 0.4±0.1*
T-030 No GFP control 26 0.3 37.0 ±7.0* 0.6±0.2*
T-030 3μg 14 35.5 19.0±2.0* 56.2±9.1*
A-020 3 μg 20 10.4 24 3.8
T-020 3 μg 14 22.7 17 31.6
X-001 3 μg 24 12.8 27 8.0
X-005 3 μg 24 16.5 20 13.7
L-029 3 μg 11 23.5 18 22.3
D-023 3 μg 15 27.2 20 14.8

Values ± SD with * represent the mean of 2 independent experiments, each with 2 from biological replicates. The other values are derived from one experiment with 2 biological replicates.

Dwivedi et al.

Dwivedi et al. BMC Biotechnology 2012 12:45   doi:10.1186/1472-6750-12-45

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