Expression of nucleofectedGpc3. Human primary calvarial suture cells (1.0 x 106) were transfected with 3 μg of mouse Gpc3 expression construct using Amaxa kit-L and the T030 program. The amplified PCR product (174 bp) shows the expression of mouse Gpc3 while no template controls (NTC) shows no bands. The positions of 100 and 200 bp markers of a DNA ladder are indicated.
Dwivedi et al. BMC Biotechnology 2012 12:45 doi:10.1186/1472-6750-12-45