Table 1

Influence of transformation factors on the frequency of transient GUS expression (%) in hypocotyls of C. roseus
Factors Variable Transient expression rate (%)a(mean ± SE)c Death rate (%)a(mean ± SE)b
A. tumefaciens infection density(OD) and duration (min) 0.5 15 53.33 ± 3.33 c 10.00 ± 2.89 b
30 78.33 ± 4.41 ab 11.67 ± 1.67 a
45 80.00 ± 2.89 a 11.67 ± 3.33 a
0.8 15 78.33 ± 3.33 b 15.00 ± 1.67 b
30 100.00 ± 0.00 a 15.00 ± 2.89 b
45 100.00 ± 0.00 a 21.67 ± 1.67 a
1.0 15 73.33 ± 1.67 b 18.33 ± 3.33 c
30 100.00 ± 0.00 a 46.66 ± 6.01 b
45 100.00 ± 0.00 a 68.33 ± 4.41 a
Co-cultivation period (d) 1 76.67 ± 4.41 b 0.00 ± 0.00 c
2 100.00 ± 0.00 a 5.00 ± 2.89 b
3 100.00 ± 0.00 a 20.00 ± 5.77 a
Acetosyringone concentration (μM) 0 13.33 ± 3.33 c 0.00 ± 0.00 cd
50 85.00 ± 2.89 ab 1.66 ± 1.66 bc
100 100.00 ± 0.00 a 5.00 ± 2.89 b
150 100.00 ± 0.00 a 46.67 ± 4.41 a

Transient expression of GUS gene was examined after co-culture. The data of death rates were collected after 10 d of co-culture. Hypocotyls were placed on MSCP1 medium.

a Transient expression rate = number of GUS+(GUS-positive) explants/number of explants × 100.

b Death rate = number of died explants/number of explants × 100.

c Values represent means for 60 explants per treatment, replicated three times. Mean ± SE in each column followed by the same letter are not significantly different according to Tukey’s multiple comparison test (P ≤ 0.05).

Wang et al.

Wang et al. BMC Biotechnology 2012 12:34   doi:10.1186/1472-6750-12-34

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