Table 1 |
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| Influence of transformation factors on the frequency of transient GUS expression (%) in hypocotyls of C. roseus | ||||
| Factors | Variable | Transient expression rate (%)a(mean ± SE)c | Death rate (%)a(mean ± SE)b | |
| A. tumefaciens infection density(OD) and duration (min) | 0.5 | 15 | 53.33 ± 3.33 c | 10.00 ± 2.89 b |
| 30 | 78.33 ± 4.41 ab | 11.67 ± 1.67 a | ||
| 45 | 80.00 ± 2.89 a | 11.67 ± 3.33 a | ||
| 0.8 | 15 | 78.33 ± 3.33 b | 15.00 ± 1.67 b | |
| 30 | 100.00 ± 0.00 a | 15.00 ± 2.89 b | ||
| 45 | 100.00 ± 0.00 a | 21.67 ± 1.67 a | ||
| 1.0 | 15 | 73.33 ± 1.67 b | 18.33 ± 3.33 c | |
| 30 | 100.00 ± 0.00 a | 46.66 ± 6.01 b | ||
| 45 | 100.00 ± 0.00 a | 68.33 ± 4.41 a | ||
| Co-cultivation period (d) | 1 | 76.67 ± 4.41 b | 0.00 ± 0.00 c | |
| 2 | 100.00 ± 0.00 a | 5.00 ± 2.89 b | ||
| 3 | 100.00 ± 0.00 a | 20.00 ± 5.77 a | ||
| Acetosyringone concentration (μM) | 0 | 13.33 ± 3.33 c | 0.00 ± 0.00 cd | |
| 50 | 85.00 ± 2.89 ab | 1.66 ± 1.66 bc | ||
| 100 | 100.00 ± 0.00 a | 5.00 ± 2.89 b | ||
| 150 | 100.00 ± 0.00 a | 46.67 ± 4.41 a | ||
Transient expression of GUS gene was examined after co-culture. The data of death rates were collected after 10 d of co-culture. Hypocotyls were placed on MSCP1 medium.
a Transient expression rate = number of GUS+(GUS-positive) explants/number of explants × 100.
b Death rate = number of died explants/number of explants × 100.
c Values represent means for 60 explants per treatment, replicated three times. Mean ± SE in each column followed by the same letter are not significantly different according to Tukey’s multiple comparison test (P ≤ 0.05).
Wang et al. BMC Biotechnology 2012 12:34 doi:10.1186/1472-6750-12-34
