Fluorescence measurements for tobacco overexpressing pporRFP (A) and pporRFPmut (codon altered) (B). Ten individual transgenic lines were selected for overexpression of each of the constructs (pporRFP and pporRFPmut). Leaf samples (youngest fully expanded leaf) from each of three individual plants were measured for each transgenic line and the average fluorescence intensity is shown. The non-transgenic tobacco (Xanthi, negative control) is denoted with a dotted line. All fluorescent measurements were normalized to the non-transgenic (Xanthi) control.
Mann et al. BMC Biotechnology 2012 12:17 doi:10.1186/1472-6750-12-17