Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants
1 Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA
2 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
3 Division of Biology, University of Tennessee, Knoxville, TN, 37996, USA
BMC Biotechnology 2012, 12:17 doi:10.1186/1472-6750-12-17Published: 3 May 2012
Additional file 1:
Schematic diagram of the T-DNA used in tobacco and Arabidopsis transformation. The vector shown is the pMDC32-tdTomato-ER. Sequence comparison of native and codon-optimized pporRFP. Underlined sequence represent ER targeting (5′) and ER retention signals (3′).
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