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Open Access Highly Accessed Research article

Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants

David GJ Mann12, Laura L Abercrombie12, Mary R Rudis1, Reggie J Millwood1, John R Dunlap3 and C Neal Stewart12*

Author Affiliations

1 Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA

2 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA

3 Division of Biology, University of Tennessee, Knoxville, TN, 37996, USA

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BMC Biotechnology 2012, 12:17  doi:10.1186/1472-6750-12-17

Published: 3 May 2012

Additional files

Additional file 1:

Schematic diagram of the T-DNA used in tobacco and Arabidopsis transformation. The vector shown is the pMDC32-tdTomato-ER. Sequence comparison of native and codon-optimized pporRFP. Underlined sequence represent ER targeting (5′) and ER retention signals (3′).

Format: RTF Size: 2.1MB Download file

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