Table 1 |
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Details of the primers used in the LAMP-BART and RT-PCR amplifications |
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Primer Type |
Orientation |
Target (5' base) |
Primer Sequence (5'-3') |
|
|
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|
Displacement |
sense |
ADH1 (7) |
CTTTGGATCGATTGGTTTC |
|
|
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|
Displacement |
antisense |
ADH1 (287) |
CCCAAAATTACTCAACG |
|
|
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|
LAMP |
sense |
ADH1 (116) |
GGTGATCAAGTGCAAAGGTCTTTTCATAAACCAAGATTAGTCAGATCAAG |
|
|
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|
LAMP |
antisense |
ADH1 (94) |
CCCCTCCGCAAATCTTCGAACAGTTTTGTAACTGGTGAAGGACTGAG |
|
|
|||
|
LOOP |
sense |
ADH1 (68) |
CGCCTTGTTTCTCCTCTGTC |
|
|
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|
LOOP |
antisense |
ADH1 (136) |
CCAAATCATCCACTCCGAGAC |
|
|
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|
Displacement |
sense |
CaMV-35 S-p (7214) |
AGGAAGGGTCTTGCG |
|
|
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|
Displacement |
antisense |
CaMV-35 S-p (7404) |
ATAAAGGAAAGGCCATCG |
|
|
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|
LAMP |
sense |
CaMV-35 S-p (7317) |
GTCTTCAAAGCAAGTGGTTTTGGATAGTGGGATTGTGCG |
|
|
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|
LAMP |
antisense |
CaMV-35 S-p (7296) |
TTCCACGATGCTCCTCGTTTTCCTCTGCCGACAGTGG |
|
|
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|
LOOP |
sense |
CaMV-35 S-p (7274) |
TCCACTGACGTAAGGG |
|
|
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|
LOOP |
antisense |
CaMV-35 S-p (7350) |
GGGGTCCATCTTTGGG |
|
|
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|
Displacement |
sense |
NOS-t (1850) |
CGCGATAATTTATCCTAGTTTG |
|
|
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|
Displacement |
antisense |
NOS-t (2053) |
CGTTCAAACATTTGGCAAT |
|
|
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|
LAMP |
sense |
NOS-t (1962) |
GCATGACGTTATTTATGAGATGGGTTTTCGCTATATTTTGTTTTCTATCGCG |
|
|
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|
LAMP |
antisense |
NOS-t (1947) |
CATGCTTAACGTAATTCAACAGTTTTTGAATCCTGTTGCCGGTC |
|
|
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|
LOOP |
sense |
NOS-t (2007) |
GATTAGAGTCCCGCAATTATAC |
|
|
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|
LOOP |
antisense |
NOS-t (1925) |
AAATTATATGATAATCATCGCAA |
|
|
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|
PCR |
sense |
ADH1 (1297) |
AATTTTGGGGAAAGCTTCGT |
|
|
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|
PCR |
antisense |
ADH1 (1369) |
TTCACCACGATTGCAGGATA |
|
|
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|
PCR |
sense |
CaMV-35 S-p (7133) |
GATTCCATTGCCCAGCTATC |
|
|
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|
PCR |
antisense |
CaMV-35 S-p (7215) |
CAACGATGGCCTTTCCTTTA |
|
|
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|
PCR |
sense |
NOS-t (1854) |
TCGTTCAAACATTTGGCAAT |
|
|
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|
PCR |
antisense |
NOS-t (1885) |
AAGACCGGCAACAGGATTC |
|
|
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|
Underscored bases of the LAMP primers are additional foreign nucleotides, introduced to link different homology segments (CAMV-35 S-p LAMP primers contain four linker bases, while the other LAMP primers only contain 3 linker bases) |
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Kiddle et al. BMC Biotechnology 2012 12:15 doi:10.1186/1472-6750-12-15 |
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