Figure 1.

Chemistry of the BART bioluminescent coupled assay. PPi liberated during DNA synthesis reacts with adenosine-5'-O-persulfate (APS) in a reaction catalysed by adenosine triphosphate sulfurylase, to form adenosine triphosphate (ATP). A recombinant thermostable firefly luciferase liberates light, CO2, PPi and adenosine monophosphate (AMP) in the presence of the substrates luciferin and oxygen.

Kiddle et al. BMC Biotechnology 2012 12:15   doi:10.1186/1472-6750-12-15
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