Chemistry of the BART bioluminescent coupled assay. PPi liberated during DNA synthesis reacts with adenosine-5'-O-persulfate (APS) in a reaction catalysed by adenosine triphosphate sulfurylase, to form adenosine triphosphate (ATP). A recombinant thermostable firefly luciferase liberates light, CO2, PPi and adenosine monophosphate (AMP) in the presence of the substrates luciferin and oxygen.
Kiddle et al. BMC Biotechnology 2012 12:15 doi:10.1186/1472-6750-12-15