Western blot indicating the presence of H5-specific antibodies in sera from mice immunised with plant-produced H5 or H5tr. Purified H5N1 HA protein (Sino Biological) was detected using sera from H5-immunised mice (lanes 3–5), or H5tr-immunised mice (lanes 6–8). Lane 1, protein ladder (Fermentas); lane 2, HA detection using anti-H5N1 HA mouse monoclonal antibody (1:5000, Abcam) and lane 9 is the negative control where sera from animals vaccinated with PBS was used (dilution 1:4000). The arrow indicates the presence of the correct band size in all the H5 and H5tr candidate vaccine sera samples.
Mortimer et al. BMC Biotechnology 2012 12:14 doi:10.1186/1472-6750-12-14