Western blot analysis of H5 and H5tr transient expression in N. benthamiana, showing accumulation in various subcellular compartments 7 days post infiltration. HA protein was detected using a primary rabbit anti-H5N1 polyclonal antibody. Crude plant extracts were analysed from plant tissue infiltrated with Agrobacterium strains carrying the following expression vectors: Lane 1, pTRAERH-H5; lane 2, pTRAERH-H5tr; lane 3, pTRAc-H5; lane 4, pTRAc-H5tr; lane 5, pTRACTP-H5; lane 6, pTRACTP-H5tr; lane 7, pTRAa-H5; lane 8, pTRAa-H5tr; lane 9, protein ladder (Fermentas). The arrow indicates the position of the H5 and H5tr proteins.
Mortimer et al. BMC Biotechnology 2012 12:14 doi:10.1186/1472-6750-12-14