Additional file 4.

Figure S1. Analysis of LMW and HMW lignin fractions. a) HPLC analysis of the LMW lignin fraction. Vanillin is indicated as a representative for the aromatic lignin monomers. Other peaks were not identified, but the absorption spectra spectra (not shown) suggested an aromatic structure; b) HPLC analysis of the high molecular weight lignin (HMW) fraction (diluted 10 times prior to HPLC measurement). Less LMW aromatic peaks were observed and vanillin was absent; c) Absorbance spectra of the dialysis buffer between 200 - 400 nm (absorption range for aromatic compounds). The absorption decreased with consecutive buffer changes, indicating that no further LMW aromatic compound were released from the HMW fraction.

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Bandounas et al. BMC Biotechnology 2011 11:94   doi:10.1186/1472-6750-11-94