Additional file 2.

pH dependence and temperature optimum of CotA activity from B. pumilus. (A) Oxidation of ABTS (■ 0.5 mM), 2,6-DMP (• 0.3 mM) and SGZ (▲ 0.05 mM) by the purified laccase as a function of pH at 25°C. Relative activities were normalized to the maximal activity achieved for each substrate at the optimum pH, which was taken as 100% (corresponds to 200, 4.3 and 5.4 U/mg for ABTS, 2,6-DMP and SGZ respectively). The oxidation by laccase as a function of temperature is given relative to the highest activity recorded, which was taken as 100%. This corresponds to 24.8 U/mg for (B) 2,6-DMP (0.3 mM, pH 7) and 857 U/mg for (C) ABTS (0.5 mM, pH 4). All data points represent mean values from triplicate determinations.

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Reiss et al. BMC Biotechnology 2011 11:9   doi:10.1186/1472-6750-11-9