Email updates

Keep up to date with the latest news and content from BMC Biotechnology and BioMed Central.

Open Access Highly Accessed Research article

Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum

Renate Reiss, Julian Ihssen and Linda Thöny-Meyer*

Author Affiliations

Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials, Lerchenfeldstr.5, 9014 St. Gallen, Switzerland

For all author emails, please log on.

BMC Biotechnology 2011, 11:9  doi:10.1186/1472-6750-11-9

Published: 25 January 2011

Additional files

Additional file 1:

Map of the constructed plasmid pBpL6 used for expression of CotA laccase from B. pumilus

Format: DOC Size: 46KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

pH dependence and temperature optimum of CotA activity from B. pumilus. (A) Oxidation of ABTS (■ 0.5 mM), 2,6-DMP (• 0.3 mM) and SGZ (▲ 0.05 mM) by the purified laccase as a function of pH at 25°C. Relative activities were normalized to the maximal activity achieved for each substrate at the optimum pH, which was taken as 100% (corresponds to 200, 4.3 and 5.4 U/mg for ABTS, 2,6-DMP and SGZ respectively). The oxidation by laccase as a function of temperature is given relative to the highest activity recorded, which was taken as 100%. This corresponds to 24.8 U/mg for (B) 2,6-DMP (0.3 mM, pH 7) and 857 U/mg for (C) ABTS (0.5 mM, pH 4). All data points represent mean values from triplicate determinations.

Format: PPT Size: 212KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data

Additional file 3:

Michaelis-Menten plots obtained with purified CotA laccase from B. pumilus. The specific activity (U/mg) was plotted versus the substrate concentration for (A) ABTS, (B) 2,6-DMP and (C) SGZ (25% (v/v) DMSO). All data points represent mean values ± standard error from triplicate determinations.

Format: PPT Size: 120KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data