Additional File 8.
Table S2. Efficiency of rhPCR at different anneal/extend temperatures. Amplification reactions were run in standard format (10 L reactions with 2.6 mU P.a. RNase H2) using 2-step PCR with anneal/extend temperatures of 50oC, 55oC, and 60oC. The SMAD7 SNP assay and “rDDDDx” blocked-cleavable primers were employed, as in Table 2.
Format: PDF Size: 211KB Download file
This file can be viewed with: Adobe Acrobat Reader
Dobosy et al. BMC Biotechnology 2011 11:80 doi:10.1186/1472-6750-11-80