Real-time amplification plots comparing the performance of blocked-cleavable primers (rhPCR) with unmodified primers. Unmodified control primers (black) and "rDDDDx" blocked-cleavable primers (blue) were used to detect a synthetic oligonucleotide amplicon in real-time qPCR format. Amplification reactions were performed in the absence of RNase H2 (left panels) or in the presence of 2.6 mU of RNase H2 (right panels). Detection was done using either SYBR® Green (top panels) or a 5'-nuclease assay with a dual-labeled hydrolysis probe (bottom panels).
Dobosy et al. BMC Biotechnology 2011 11:80 doi:10.1186/1472-6750-11-80