Table 1

Differences in mean RNA substrate ligated by T4 Rnl2tr mutants over time.

Time (h)

Rnl2tr + MBP

K227Q

R55K

K225R

R55K K227Q

K225R K227Q

R55K K225R K227Q


1

NS

*

NS

**

NS

NS

NS


2

NS

***

NS

***

NS

NS

**


3

NS

***

NS

***

NS

**

***


6

NS

***

NS

***

NS

**

***


9

NS

***

NS

***

NS

NS

***


12

NS

**

NS

***

NS

NS

**


24

NS

NS

NS

***

NS

NS

NS


Mean levels of RNA substrate ligated by T4 Rnl2tr mutants that are significantly different are lower than the corresponding WT T4 Rnl2tr values (see Fig 6). NS = no significant difference detected, * = means differ p < 0.05, ** = means differ p < 0.01, *** = means differ p < 0.001 as determined by two-way ANOVA followed by Bonferroni post-tests for comparison to T4 Rnl2tr.

Viollet et al. BMC Biotechnology 2011 11:72   doi:10.1186/1472-6750-11-72

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