Table 1 |
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|
Effect of preparation conditions on lipase immobilization efficiency and activity of lipase immobilized PD-MNP |
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|
Dopamine (mg/ml) |
PD-MNP (mg) |
Lipase (mg) |
Bound Lipase (mg) |
Activity (U) |
Specific Activity (U/mg lipase) |
|
|
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|
0 |
10 |
10 |
3.16 ± 0.52 |
9.75 ± 2.28 |
3.09 ± 0.16 |
|
0.25 |
10 |
10 |
6.96 ± 0.49 |
18.19 ± 2.90 |
2.62 ± 0.13 |
|
0.5 |
10 |
10 |
7.81 ± 0.62 |
21.08 ± 2.49 |
2.70 ± 0.17 |
|
1.0 |
10 |
10 |
8.26 ± 0.48 |
28.81 ± 3.08 |
3.49 ± 0.17 |
|
2.5 |
10 |
10 |
9.17 ± 0.58 |
59.00 ± 3.39 |
6.44 ± 0.38 |
|
3.75 |
10 |
10 |
9.37 ± 0.55 |
62.50 ± 3.04 |
6.67 ± 0.37 |
|
5.0 |
10 |
10 |
9.46 ± 0.57 |
64.89 ± 2.69 |
6.86 ± 0.40 |
|
2.5 |
10 |
5 |
4.29 ± 0.22 |
37.65 ± 2.52 |
8.78 ± 0.33 |
|
Free lipase |
10 |
118.76 ± 4.13 |
11.88 ± 0.41 |
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|
|
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|
Effect of preparation conditions on lipase immobilization efficiency and activity of lipase immobilized PD-MNP (n = 3). |
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|
Ren et al. BMC Biotechnology 2011 11:63 doi:10.1186/1472-6750-11-63 |
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