Table 1

Effect of preparation conditions on lipase immobilization efficiency and activity of lipase immobilized PD-MNP

Dopamine

(mg/ml)

PD-MNP

(mg)

Lipase (mg)

Bound Lipase (mg)

Activity

(U)

Specific Activity (U/mg lipase)


0

10

10

3.16 ± 0.52

9.75 ± 2.28

3.09 ± 0.16

0.25

10

10

6.96 ± 0.49

18.19 ± 2.90

2.62 ± 0.13

0.5

10

10

7.81 ± 0.62

21.08 ± 2.49

2.70 ± 0.17

1.0

10

10

8.26 ± 0.48

28.81 ± 3.08

3.49 ± 0.17

2.5

10

10

9.17 ± 0.58

59.00 ± 3.39

6.44 ± 0.38

3.75

10

10

9.37 ± 0.55

62.50 ± 3.04

6.67 ± 0.37

5.0

10

10

9.46 ± 0.57

64.89 ± 2.69

6.86 ± 0.40

2.5

10

5

4.29 ± 0.22

37.65 ± 2.52

8.78 ± 0.33

Free lipase

10

118.76 ± 4.13

11.88 ± 0.41


Effect of preparation conditions on lipase immobilization efficiency and activity of lipase immobilized PD-MNP (n = 3).

Ren et al. BMC Biotechnology 2011 11:63   doi:10.1186/1472-6750-11-63

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