The effect of temperature on the activity of free and immobilized lipase. Activity was measured at 37°C after treatment for 1 hour in phosphate buffer (10 mM, pH 7.0) at the temperatures indicated. The PD-MNPs were prepared using 2.5 mg/ml initial dopamine and lipase immobilization performed using 5 mg/ml initial lipase and 10 mg/ml PD-MNPs concentration.
Ren et al. BMC Biotechnology 2011 11:63 doi:10.1186/1472-6750-11-63