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Open Access Highly Accessed Research article

Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating

Yuhong Ren1*, Jose G Rivera23, Lihong He23, Harsha Kulkarni23, Dong-Keun Lee23 and Phillip B Messersmith234567*

Author Affiliations

1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

2 Biomedical Engineering Department, Northwestern University, 2145 Sheridan Road, Evanston 60208, USA

3 Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston 60208, USA

4 Chemical and Biological Engineering Department, Northwestern University, 2145 Sheridan Road, Evanston 60208, USA

5 Materials Science and Engineering Department, Northwestern University, 2145 Sheridan Road, Evanston 60208, USA

6 Institute for Bionanotechnology in Medicine, Northwestern University, 2145 Sheridan Road, Evanston 60208, USA

7 Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2145 Sheridan Road, Evanston 60208, USA

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BMC Biotechnology 2011, 11:63  doi:10.1186/1472-6750-11-63

Published: 8 June 2011

Abstract

Background

Immobilization of lipase on appropriate solid supports is one way to improve their stability and activity, and can be reused for large scale applications. A sample, cost- effective and high loading capacity method is still challenging.

Results

A facile method of lipase immobilization was developed in this study, by the use of polydopamine coated magnetic nanoparticles (PD-MNPs). Under optimal conditions, 73.9% of the available lipase was immobilized on PD-MNPs, yielding a lipase loading capacity as high as 429 mg/g. Enzyme assays revealed that lipase immobilized on PD-MNPs displayed enhanced pH and thermal stability compared to free lipase. Furthermore, lipase immobilized on PD-MNPs was easily isolated from the reaction medium by magnetic separation and retained more than 70% of initial activity after 21 repeated cycles of enzyme reaction followed by magnetic separation.

Conclusions

Immobilization of enzyme onto magnetic iron oxide nanoparticles via poly-dopamine film is economical, facile and efficient.