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Open Access Highly Accessed Methodology article

Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing

Kerry A Lutz12*, Wenqin Wang1, Anna Zdepski1 and Todd P Michael3

Author Affiliations

1 Rutgers, The State University of New Jersey, Department of Plant Biology and Pathology, The Waksman Institute of Microbiology, Piscataway, NJ 08854, USA

2 Current Address: Farmingdale State College, Ward Hall, Room 307, 2350 Broadhollow Road, Farmingdale, NY 11735, USA

3 Monsanto Company, 800 North Lindbergh Blvd., Creve Coeur, Missouri 63167, USA

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BMC Biotechnology 2011, 11:54  doi:10.1186/1472-6750-11-54

Published: 20 May 2011

Additional files

Additional file 1:

qPCR primers. A list of primers used for qPCR analysis.

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Additional file 2:

qPCR results of Arabidopsis thaliana nuclei and CTAB isolated DNA with nuclear (Gi), mitochondrial (cox1) and chloroplast (rps18) primers. qPCR results for Arabidopsis thaliana. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated for each primer pair qCR reaction and number of organelles per diploid genome.

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Additional file 3:

qPCR results of Sorghum bicolor nuclei and CTAB isolated DNA with nuclear (Gi), mitochondrial (cox1) and chloroplast (psbA) primers. qPCR results for Sorghum bicolor. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated efficiencies for each primer pair qPCR reaction and number of organelles per diploid genome.

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Additional file 4:

qPCR results of Zea mays nuclei and CTAB isolated DNA with nuclear (Gi), mitochondrial (cox1) and chloroplast (psbA) primers. qPCR results for Zea mays. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated efficiencies for each primer pair qPCR reaction and number of organelles per diploid genome.

Format: DOC Size: 62KB Download file

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Additional file 5:

qPCR results of Vaccinium macrocarpon nuclei and CTAB isolated DNA with nuclear (Dfr2), mitochondrial (matR) and chloroplast (rbcL) primers. qPCR results for Vaccinium macrocarpon. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated efficiencies for each primer pair qPCR reaction and number of organelles per diploid genome.

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Additional file 6:

qPCR results of Lemna gibba nuclei and CTAB isolated DNA with nuclear (Gi) and chloroplast (matK) primers. qPCR results for Lemna gibba. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated efficiencies for each primer pair qPCR reaction and number of organelles per diploid genome.

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Additional file 7:

qPCR results of Spirodela polyrhiza nuclei and CTAB isolated DNA with nuclear (Gi) and chloroplast (rbcL) primers. qPCR results for Spirodela polyrhiza. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated efficiencies for each primer pair qPCR reaction and number of organelles per diploid genome.

Format: DOC Size: 49KB Download file

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Additional file 8:

qPCR results of Genlisea aurea nuclei and CTAB isolated DNA with nuclear (Gi), mitochondrial (cox1) and chloroplast (rps16) primers. qPCR results for Genlisea aurea. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated efficiencies for each primer pair qPCR reaction and number of organelles per diploid genome.

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Additional file 9:

qPCR results of Brachypodium distachyon nuclei and CTAB isolated DNA with nuclear (Gi) and chloroplast (psbA) primers. qPCR results for Brachypodium distachyon. Table contains the diluted DNA concentrations, with the corresponding Ct values as well as the calculated efficiencies for each primer pair qPCR reaction and number of organelles per diploid genome.

Format: DOC Size: 51KB Download file

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Open Data