Table 4

Hydraulic conductivity (Lp) and ethylene glycol permeability (PEG) of X. laevis oocytes expressing wild-type (WT) DrAqp3b, two DrAqp3b mutants or HsAQP3, in hypertonic ethylene glycol solution

Lp (μm/min/atm)

PEG (x 10-3 cm/min)


Water

0.36 ± 0.02 a

0.47 ± 0.03 a

DrAqp3b-WT (1 ng)

2.15 ± 0.14 c

17.59 ± 0.83 d

DrAqp3b-H53A/G54H/T85A (1 ng)

1.55 ± 0.13 b

13.06 ± 0.72 c

DrAqp3b-T85A (1 ng)

2.64 ± 0.15 d

25.49 ± 1.56 e

HsAQP3 (1 ng)

1.31 ± 0.08 b

10.73 ± 0.59 b

DrAqp3b-WT (20 ng)

2.63 ± 0.07 d

40.06 ± 1.22 c

DrAqp3b-H53A/G54H/T85A (20 ng)

2.27 ± 0.07 c

36.58 ± 1.74 c

DrAqp3b-T85A (20 ng)

3.24 ± 0.08 e

53.91 ± 2.55 d

HsAQP3 (20 ng)

1.96 ± 0.08 b

22.24 ± 0.95 b


Values (mean ± SEM) for water- and aquaporin-injected (1 or 20 ng cRNA) oocytes are calculated from the data in Figure 6B and C. For each cRNA dose, data with different superscript are statistically significant (ANOVA, p < 0.05).

Chauvigné et al. BMC Biotechnology 2011 11:34   doi:10.1186/1472-6750-11-34

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