Table 1

Cryoprotectant permeability (PS) of X. laevis oocytes expressing human AQP3 or zebrafish GLPs in isotonic solutions at different pH containing 160 mM of solutes

PGly (x 10-3 cm/min)

PPG (x 10-3 cm/min)

PEG (x 10-3 cm/min)




pH 6.0

pH 7.5

pH 8.5

pH 6.0

pH 7.5

pH 8.5

pH 6.0

pH 7.5

pH 8.5


HsAQP3

9.95 ± 1.17 a

(1.64 ± 0.29)

35.22 ± 3.48 c

(2.07 ± 0.32)

49.62 ± 5.96 e

(3.13 ± 0.38)

10.05 ± 1.64 a

(2.47 ± 0.29)

28.93 ± 3.62 c

(3.13 ± 0.34)

38.14 ± 2.87 cd

(5.23 ± 0.56)

18.34 ± 2.32 b

(1.97 ± 0.31)

32.38 ± 2.87 c

(4.30 ± 0.40)

36.53 ± 3.28 c

(4.89 ± 0.62)

DrAqp3a

2.21 ± 0.40 a

(1.45 ± 0.20)

4.64 ± 0.97b

(2.10 ± 0.30)

32.64 ± 4.53 d

(3.33 ± 0.48)

5.73 ± 0.91 b

(2.41 ± 0.21

16.09 ± 1.86 c

(2.64 ± 0.28)

71.08 ± 6.85 e

(5.41 ± 0.46)

3.76 ± 0.69 ab

(1.83 ± 0.29)

20.63 ± 3.18 c

(3.70 ± 0.66)

90.04 ± 6.02 f

(3.77 ± 0.39)

DrAqp3b 1

1.71 ± 0.43 a

(1.11 ± 0.25)

12.81 ± 1.24 b

(1.47 ± 0.31)

57.37 ± 3.03 d

(2.93 ± 0.63)

1.92 ± 0.42 a

(1.54 ± 0.33)

28.27 ± 2.51 c

(3.77 ± 0.37)

70.08 ± 4.48 e

(7.84 ± 0.98)

1.46 ± 0.29 a

(1.15 ± 0.71)

79.22 ± 5.70 e

(4.50 ± 0.50)

_

DrAqp7

nd

97.99 ± 7.16 c

(3.85 ± 0.55)

nd

nd

40.76 ± 3.37 a

(3.34 ± 0.35)

nd

Nd

50.55 ± 2.81 b

(4.53 ± 0.57)

nd

DrAqp9a

nd

27.58 ± 1.85 a

(1.76 ± 0.16)

nd

nd

29.40 ± 3.19 a

(5.06 ± 0.37)

nd

Nd

61.62 ± 4.45 b

(7.75 ± 0.60)

nd

DrAqp9b

nd

52.48 ± 3.54 b

(1.92 ± 0.24)

nd

nd

31.27 ± 4.39 a

(3.94 ± 0.38)

nd

Nd

56.03 ± 4.30 b

(9.24 ± 0.70)

nd

DrAqp10a

nd

31.05 ± 3.01 b

(1.60 ± 0.23)

nd

nd

13.02 ± 1.45 a

(4.45 ± 0.41)

nd

Nd

19.14 ± 1.17 a

(6.99 ± 0.61)

nd

DrAqp10b

1.15 ± 0.16 a

(1.29 ± 0.21)

2.29 ± 0.32 b

(1.71 ± 0.26)

17.48 ± 2.25 ef

(2.84 ± 0.52)

2.45 ± 0.50 bc

(2.04 ± 0.30)

3.66 ± 0.51 c

(2.59 ± 0.30)

13.21 ± 1.35 e

(4.59 ± 0.56)

3.30 ± 0.99 bc

(1.93 ± 0.30)

6.70 ± 0.82 d

(3.67 ± 0.74)

18.58 ± 1.26 f

(3.77 ± 0.41)


Data are the means ± SEM (n = 10-12 oocytes per treatment) of 3-4 experiments performed on different batches of oocytes. Due to natural variations in oocyte permeability between batches of oocytes, the PS of water-injected oocytes (controls) is shown in parenthesis for each aquaporin. Oocytes were injected with 1 ng (DrAqp3b, -7, -9a, -9b, -10a, -1a, or HsAQP3) or 10 ng (DrAqp3a and -10b) cRNA, or with 50 nl of water. Data from the same row with different superscript are statistically significant (ANOVA, p < 0.05). nd, not determined.

1 No data available for PEG at pH 8.5 because DrAqp3b-expressing oocytes burst in 160 mM ethylene glycol.

Chauvigné et al. BMC Biotechnology 2011 11:34   doi:10.1186/1472-6750-11-34

Open Data