Table 3

List of test proteins used to validate the groEL1ΔC expression strain

ORF

Annotation

Description

Expressed ...

Mol. Mass (kDa)


Rv2109c

Rv2110c

PrcA

PrcB

α- and β-subunit of the mycobacterial proteasome (α7β7β7α7 subunit organisation)

Using native operon content, producing a 730 kDa multimeric complex

26.8

30.3


Rv3285

Rv3280

AccA3

AccD5

α- and β-subunit from acyl-CoA carboxylase AccD5-AccA3 complex (α3β3β3α3 subunit organisation)

As monomeric proteins, mixed to form a acyl-CoA carboxylase complex of 740 kDa

63.8

59.4


Rv3874

Rv3875

CFP10

ESAT6

Potential virulence factor CFP10-ESAT6 complex

Using native operon content, producing a heterodimeric (1:1) complex

10.8

9.9


Rv2523c

ACPS

Holo-acyl-carrier protein synthase

As monomeric protein

14


Noens et al. BMC Biotechnology 2011 11:27   doi:10.1186/1472-6750-11-27

Open Data