Open Access Highly Accessed Research article

Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain

Elke E Noens1*, Chris Williams1, Madhankumar Anandhakrishnan1, Christian Poulsen2, Matthias T Ehebauer1 and Matthias Wilmanns1

Author Affiliations

1 European Molecular Biology Laboratory (EMBL), Hamburg Outstation, c/o DESY, Building 25a, Notkestraße 85, 22603 Hamburg, Germany

2 The Scripps Research Institute, La Jolla, 92037, USA

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BMC Biotechnology 2011, 11:27  doi:10.1186/1472-6750-11-27

Published: 25 March 2011

Additional files

Additional file 1:

GroEL1 is absent from an AccD5 protein sample derived from M. smegmatis groEL1ΔC. Results of peptide mass fingerprinting analysis of samples excised from SDS-PAGE gel (Figure 3, boxes). Shown in red are the peptides that could be identified. (a) Sample derived from M. smegmatis mc2155. (b) Sample derived from M. smegmatis groEL1ΔC.

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