Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain
1 European Molecular Biology Laboratory (EMBL), Hamburg Outstation, c/o DESY, Building 25a, Notkestraße 85, 22603 Hamburg, Germany
2 The Scripps Research Institute, La Jolla, 92037, USA
BMC Biotechnology 2011, 11:27 doi:10.1186/1472-6750-11-27Published: 25 March 2011
Additional file 1:
GroEL1 is absent from an AccD5 protein sample derived from M. smegmatis groEL1ΔC. Results of peptide mass fingerprinting analysis of samples excised from SDS-PAGE gel (Figure 3, boxes). Shown in red are the peptides that could be identified. (a) Sample derived from M. smegmatis mc2155. (b) Sample derived from M. smegmatis groEL1ΔC.
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