Table 2 

Kinetics parameters of NOX towards different cofactors. 

Km (mM) 
kcat (s1) 
kcat/Km × 10^{6}(M1*s1) 



NADH^{a} 
2.1 ± 0.4 
15.6 ± 0.7 
7.4 


FAD^{b} 
34.1 ± 6.0 
 
 


FMN^{b} 
42.8 ± 5.1 
 
 


The steadystate kinetics parameters were calculated at pH 7 and 25°C (See methods). Activities were adjusted to a nonlinear regression.^{a }Kinetics parameters for NADH were calculated using 50 μM FAD.^{b }Kinetics parameters for flavin cofactors were calculated using 10 μM NADH. 

RochaMartín et al. BMC Biotechnology 2011 11:101 doi:10.1186/1472675011101 