Table 2

Kinetics parameters of NOX towards different cofactors.

Km

(mM)

kcat

(s-1)

kcat/Km

× 106(M-1*s-1)


NADHa

2.1 ± 0.4

15.6 ± 0.7

7.4


FADb

34.1 ± 6.0

-

-


FMNb

42.8 ± 5.1

-

-


The steady-state kinetics parameters were calculated at pH 7 and 25°C (See methods). Activities were adjusted to a non-linear regression.a Kinetics parameters for NADH were calculated using 50 μM FAD.b Kinetics parameters for flavin cofactors were calculated using 10 μM NADH.

Rocha-Martín et al. BMC Biotechnology 2011 11:101   doi:10.1186/1472-6750-11-101

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