Table 1

Purification of NADH oxidase from E.coli.

Entrya

Protein (mg/ml)

Specific Activity (U/mg)b

Purification factor

Purification yield (%)


1

26.29

0.25

1

100


2

6.48

1.88

7.5

100


3

3.3

3.69

14.7

80


4

2.93

4.15

16.6

70


aEntry 1: Crude extract. Entry 2: Heat treatment (80°C) for 45 min. Entry 3: Heat treatment (80°C 45 minutes) and further incubation with PEI-ag for 30 min. Entry 4: Heat treatment (80°C 45 minutes) and sequential b activity at 65°C, pH 7 without exogenous FAD/FMN.

Rocha-Martín et al. BMC Biotechnology 2011 11:101   doi:10.1186/1472-6750-11-101

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