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Open Access Correction

Correction: Analysis of conditional gene deletion using probe based Real-Time PCR

Britta Weis1*, Joachim Schmidt2, Frank Lyko1 and Heinz G Linhart3

Author Affiliations

1 Division of Epigenetics, German Cancer Research Center, Heidelberg, Germany

2 Division of Translational Oncology, German Cancer Research Center, Heidelberg, Germany

3 Department of Gastroenterology and Hepatology, University Hospital Freiburg, Hugstetter Str. 49, 79106 Freiburg, Germany

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BMC Biotechnology 2010, 10:90  doi:10.1186/1472-6750-10-90

Published: 21 December 2010

Abstract

Following publication of this article [1] the authors noticed that an incorrect probe reference was cited on page 3, 4, 5 and 6 ("UP #69, Roche Applied Science"). The correct probe that was used for the 1lox/2lox allele ratio analysis in the paper is as follows

Probe for 1lox/2lox allele quantification:

5'-6-FAM-atAaCtTCgtatagCATaCattatac-BHQ-1 -3'

(uppercase letters = LNA bases)

Manufacturer: EUROGENTEC, Seraing, Belgium

All other information and reaction conditions in the paper are correct as stated.