|
Resolution: standard / high Figure 3.
Affinity determination of RAbDMvIII by Saturation ELISA. Calibration curve of the binding of RAbDMvIII and RAbvIII recombinant antibodies to coated EGFRvIII in the ELISA. A) The concentration range
for RAbDMvIII and RAbvIII was 20 to 0.02 ug/ml (x axis) and the absorbance was read at 450 nm (Y axis). The
assay was analyzed twice. B) Saturation ELISA shows the Scatchard plot of the binding
of RAbDMvIII to EGFRvIII. The resulting data is plotted as ratio of α/c (y axis) as function of
alpha (x axis) where α is the fraction of bound antibody sites and c is the concentration
of free antigen. The slope is -K which is the affinity constant of antibody and that
is 1.7 × 107 liters/mole (M-1).
Gupta et al. BMC Biotechnology 2010 10:72 doi:10.1186/1472-6750-10-72 |
