Schematic of the RAbDMvIII antibody. The genes for the anti-EGFRvIII scFvs were cloned into the pBudCE4.1 dicistronic vector. The Ig k-chain signal sequence at the 5' end of each sequence allowed the secretion of the molecule in the culture media. "Knobs-into-holes" configuration and the two cysteine residues at the 3' end of each CH3 domain stabilize the construct. Sequence corresponding to a V5 epitope was cloned at the 3' end of one anti-EGFR scFvs and the other anti-EGFR scFv has Myc as well as a 6xHis tag at 3'end.
Gupta et al. BMC Biotechnology 2010 10:72 doi:10.1186/1472-6750-10-72