Research article
Characterization of single chain antibody targets through yeast two hybrid
-
* Corresponding author: Franck Perez Franck.Perez@curie.fr
1 Institut Curie - Research Center, 26 rue d'Ulm, 75248 Paris cedex 05, France
2 CNRS, UMR144, 26 rue d'Ulm, 75248 Paris, cedex 05, France
3 U830 INSERM, 26, rue d'Ulm, 75248 Paris cedex 05, France
4 Hybrigenics Inc., 3-5 impasse Reille, 75014 Paris, France
5 Drexel University School of Medicine, Division of Infectious Diseases &HIV Medicine, 245N 15th Street, NCB 6th floor, Philadelphia, PA 19102, USA
6 Université Joseph Fourier (Grenoble) Laboratoire de Spectrométrie Physique, CNRS, BP87 (UMR5588) 140 Av. de la physique, 38402 Saint Martin d'Hères, France
7 Institut Pasteur, Membrane Traffic and Cell Division Lab, CNRS URA2582,26 rue du Dr. Roux, 75015 Paris, France
BMC Biotechnology 2010, 10:59 doi:10.1186/1472-6750-10-59
Published: 22 August 2010Additional files
Additional file 1:
AA2 Two Hybrid Screen Results using human cDNA library. A table listing the identity of all the hits recovered in the two-Hybrid screen using AA2 as a bait against a human cDNA library. The table presents the name and accession number of each prey (identified by alignment, see materials and methods), the nucleotide start and stop of the insert, whether it is in frame or out of frame (OOF), its sense in the prey vector and the calculated PBS score (see materials and methods).
Format: PDF Size: 42KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 2:
AA2 Two Hybrid Screen Results using drosophila cDNA library. A table listing the identity of all the hits recovered in the two-Hybrid screen using AA2 as a bait against a drsophila cDNA library. The table presents the name and accession number of each prey (identified by alignment, see materials and methods), the nucleotide start and stop of the insert, whether it is in frame or out of frame (OOF), its sense in the prey vector and the calculated PBS score (see materials and methods).
Format: PDF Size: 44KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 3:
ROF7 Two Hybrid Screen Results using human cDNA library. A table listing the identity of all the hits recovered in the two-Hybrid screen using ROF7 as a bait against a human cDNA library. The table presents the name and accession number of each prey (identified by alignment, see materials and methods), the nucleotide start and stop of the insert, whether it is in frame or out of frame (OOF), its sense in the prey vector and the calculated PBS score (see materials and methods).
Format: PDF Size: 70KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 4:
TA10 Two Hybrid Screen Results using human cDNA library. A table listing the identity of all the hits recovered in the two-Hybrid screen using TA10 as a bait against a human cDNA library. The table presents the name and accession number of each prey (identified by alignment, see materials and methods), the nucleotide start and stop of the insert, whether it is in frame or out of frame (OOF), its sense in the prey vector and the calculated PBS score (see materials and methods).
Format: PDF Size: 53KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 5:
SF9 Two Hybrid Screen Results using human cDNA library. A table listing the identity of all the hits recovered in the two-Hybrid screen using SF9 as a bait against a human cDNA library. The table presents the name and accession number of each prey (identified by alignment, see materials and methods), the nucleotide start and stop of the insert, whether it is in frame or out of frame (OOF), its sense in the prey vector and the calculated PBS score (see materials and methods).
Format: PDF Size: 98KB Download file
This file can be viewed with: Adobe Acrobat Reader