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Novel strong tissue specific promoter for gene expression in human germ cells

Denis Kuzmin1, Elena Gogvadze1, Roman Kholodenko1, Dawid P Grzela2, Maxim Mityaev1, Tatyana Vinogradova1, Eugene Kopantzev1, Galina Malakhova1, Maria Suntsova1, Dmitry Sokov3, Zoltán Ivics2 and Anton Buzdin1*

  • * Corresponding author: Anton Buzdin bu3din@mail.ru

  • † Equal contributors

Author Affiliations

1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

2 Max Delbrück Center for Molecular Medicine, Berlin, Germany

3 P.A. Herzen Moscow Oncological Research Institute, Moscow, Russia

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BMC Biotechnology 2010, 10:58  doi:10.1186/1472-6750-10-58

Published: 17 August 2010

Additional files

Additional file 1:

Figure S1: Schematic representation of NDUFV1 upstream region. 3665 bp-long NUS sequence contains highly conserved 91 bp-long genomic fragment located exactly upstream NDUFV1 transcriptional start site. At the 5' terminus of NUS there is an insert of HERV-K (HML-2) solitary LTR.

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Additional file 2:

Figure S2: Comparison of herpes virus thymidine kinase promoter - driven renilla luciferase activities in cell lines Tera-1, NGP127, HepG2, A549 and HEK293. The column height reflects the mean value of detected luciferase activity from at least six transfection experiments, and error bars indicate the value of a standard error. Each experiment was made at least in quadruplicate.

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Additional file 3:

Figure S3: Comparison of mNUS and CMV promoter activities in cell lines Tera-1 and HEK293. The column height reflects the mean value of relative luciferase activity from at least four transfections, and error bars indicate the value of a standard error.

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Additional file 4:

Figure S4: Schematic map of the putative transcriptional factor binding sites within the 3665 bp-long entire NUS region. HERV-K(HML-2) LTR sequence and 91 bp-long deletion are shown by magenta boxes. Signs above/below represent putative transcription factor binding sites identified in the sense/antisense orientations, respectively. Large red circle represent putative SRY/SOX3 binding site perfectly matching the consensus sequence (ACAAAACA), small circles - sites with single nucleotide mismatches. The detailed data on transcriptional factor binding sites is presented on the additional table 1.

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