Dissociation of chimera in the transgenic tobacco line T3. The chimera was divided in single-node explants and subcultured for 9 additional cycles. Then three different shoots (S1, S4 and S5) were selected because of their good rooting system and dark green leaves and their DNA was extracted from the apical (AL) and basal (BL) leaves. Values are means from four to six independent DNA extractions from each shoot (or eight to twelve from each shoot/position combination).
Faize et al. BMC Biotechnology 2010 10:53 doi:10.1186/1472-6750-10-53