Detection of chimerism in the transformed apricot lines HN507-31, HPinAB12 and HPinB3. Plants were transformed with the binary vector pBin19-35SGusintron carrying the nptII transgene for kanamycin selection. A) DNA was independently extracted from apical (AL) and basal leaves (BL) of five different shoots of the line HN507-31, and used for relative determination of the amount of nptII using quantitative, real-time PCR. B) DNA was also independently extracted from apical leaves (AL) and from roots (R) of three different shoots of each of the transgenic lines HPinAB12 (blue) and HPinB3 (green), and used for real-time PCR.
Faize et al. BMC Biotechnology 2010 10:53 doi:10.1186/1472-6750-10-53