Table 2

Detailed overview of characterized antibodies

Epitope

Antibody

Clonality

Company

Product N°

Concentration

Optimal dilution for IHC based on current study

Optimal concentration (μg/ml) for IHC based on current study


3flag and flag

Anti-flag polyclonal1

Polyclonal

Sigma

F7425

0.8 mg/ml

1/5000

0.16

FlagM21

Monoclonal

Sigma

F3165

5 mg/ml

1/12500

0.4

V5

Anti-V53

Monoclonal

Invitrogen

R960-25

1.07 mg/ml

1/12500

0.0856

Myc

Anti-Myc2

Polyclonal

Polyclonal

Upstate

06-549

1 mg/ml

1/5000

0.2

9E103

Monoclonal

Santa Cruz

Sc-40

0.2 mg/ml

1/1000

0.2

HA

HA 113

Monoclonal

Covance

MMS-101R

2-3 mg/ml

1/25000

0.1

12CA53

Monoclonal

Roch Applied

11 583 816 001

0.4 mg/ml

1/5000

0.08

AU1

Anti-AU13

Monoclonal

Covance

MMS-130R

5-7 mg/ml

1/5000

1.2

GFP

Anti-eGFP4

Polyclonal

Polyclonal

In-house

-

-

1/10000

-


1 affinity purified IgGs using a column bearing the inmmunizing peptide

2 affinity purified IgGs using a column bearing protein A

3 purification method not specified

4 not purified

In order to compare antibody dilutions, the optimal dilutions (based on current study) for IHC reckoned with the antibody concentration are mentioned in this table.

Lobbestael et al. BMC Biotechnology 2010 10:16   doi:10.1186/1472-6750-10-16

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