Timeline, pressure changes and instantaneous frequency plot of an isolated guinea pig bladder after stimulation with arecaidine showing the reversibility of the indomethacin effect by PGE2. A. an outline of another typical experiment. The same protocol as described in Figure 3 was followed. To check the reversibility, at the end of the experiment, 1 μM PGE2 is added in to the organ bath and a last arecaidine stimulation is conducted. The first arecaidine stimulation is marked as (1) followed by the stimulation with presence of indomethacin (2) followed by stimulation after a wash step (3). At the end an arecaidine stimulation is shown after adding PGE2 to the system (4). B. Each of the arecaidine responses shown in panel A in an enlarged scale, starting from the left, the first trace shows the basic arecaidine response (1), the second trace shows the previously shown effect of the inhibition of PG production (2). The third trace shows the effect of washing the organ bath with fresh Krebs’ before stimulation with arecaidine (3). The last trace from the right shows the effect of addition of exogenous PGE2 (4). C. Instantaneous frequency plot comparing the four conditions described in panel A.
Rahnama’i et al. BMC Urology 2013 13:8 doi:10.1186/1471-2490-13-8