The timeline of the experiments. Pressure recordings started with stimulation of the guinea pig bladder by adding the cholinergic agonist arecaidine to the organ bath with Krebs’ solution in which the isolated guinea pig bladder was kept. After 15 minutes the Krebs’ solution is renewed (wash step). The COX inhibitor indomethacin was added 15 minutes after the first wash step. This was then followed by two sets of arecaidine stimulation and wash steps each separated by 15 minutes. In four experiments PGE2 was added to the organ 15 minutes after the third wash step which was then followed by a set of arecaidine stimulation and wash step, 30 minutes later.
Rahnama’i et al. BMC Urology 2013 13:8 doi:10.1186/1471-2490-13-8