Diclofenac induced the tumor growth delay and enhanced the effect of RT in xenograft mouse model of LNCaP-COX-2. The xenograft mice bearing LNCaP-COX-2 tumor were treated with topical administration of diclofenac gel onto mice skin with cotton swab once a day (day 1–36). The tumors in RT alone group and combination group were exposed to IR with a single dose of 3 Gy on day 3. Tumor volume was measured with calipers and calculated as 0.52LW2. (A) Tumor volume was significantly reduced in combination group. (B) Immunohistochemical staining for COX-2 and Ki-67 was conducted. The expression of COX-2 decreased in diclofenac group and combination group compared with control group, while the expression of COX-2 increased in RT group. The expression of Ki-67 significantly decreased in diclofenac group and combination group. Original magnification, x200.
Inoue et al. BMC Urology 2013 13:1 doi:10.1186/1471-2490-13-1