Effects of diclofenac on LNCaP-COX-2 cells and LNCaP-Neo. Cells were seeded in 96-well plate, and treated with indicated concentration of diclofenac for 72 hours. Cytotoxicity was determined by WST-8 assay. Diclofenac reduced cell viability in both LNCaP-COX-2 cells and LNCaP-Neo cells in a dose-dependent manner. LNCaP-COX-2 cells significantly exhibited higher sensitivity to diclofenac than LNCaP-Neo cells. Cell viability at 10 μM in LNCaP-COX-2 and LNCaP-Neo was 74.0% and 95.7% (p = 0.0094), 51.6% and 73.8% at 50 μM (p < 0.0001), 31.7% and 45.7% at 100 μM (p < 0.0001). Absorbance values were normalized to values for vehicle only treated cells (control), and the results are expressed as the mean ± SD of sextuplet samples.
Inoue et al. BMC Urology 2013 13:1 doi:10.1186/1471-2490-13-1