NIS-Elements BR 3.00 software image conversion for quantification of total epithelial, subepithelial interstitial, and muscularis layers of mouse bladder sections.A) Images of H&E-stained mouse bladder sections (shown on left) were generated at 20X using a Nikon Eclipse TE300 Inverted Microscope and the area of specific tissue layers measured using NIS-Elements BR 3.00 Imaging Software with custom macros (resulting image shown on right). B) These images were then used to determine the ratio of epithelial area to total bladder cross-sectional area for mice in each treatment group for all time points using NIS-Elements BR 3.00 Imaging Software. Data were combined from five experiments and analyzed using two-way ANOVA; *p < .0001; **p < .01; ***p < .02; ****p < .04. Treatment group: PBS □; inactive control peptide ■; as-APF ░.
Keay et al. BMC Urology 2012 12:17 doi:10.1186/1471-2490-12-17