Proteasome inhibition causes an increase in p21. (A) UMUC-3 cells were serum starved for 24 hours and then treated with 1 μM and 10 μM MG-132 over a 180 minute time course. Cells were lysed and immunoblotted for p21 and tubulin. (B) Quantitation of relative p21 levels normalized to tubulin after 180 minutes treatment with 1 μM MG-132 is shown for UMUC-3 and UMUC-14 cells. Four replicates were tested. Error bars represent S.E.M.
Yohn et al. BMC Urology 2011 11:19 doi:10.1186/1471-2490-11-19