EGF induces p21 in a PI3-kinase and AKT dependent manner. (A) UMUC-3 cells were pre-treated for one hour with PI3-kinase inhibitor LY294002 (30 μM) or AKT inhibitor AKTi-1/2 (10 μM) and then stimulated with EGF over a 180 minute time course. Cells were harvested at times shown and probed for expression of p21 and phosphorylated AKT serine 473. (B) Quantitation of relative p21 signal normalized to tubulin after 180 minute EGF treatment in the presence or absence of pretreatment with LY294002 or Akti-1/2 is shown. Four replicates of each treatment were tested. Error bars represent S.E.M.
Yohn et al. BMC Urology 2011 11:19 doi:10.1186/1471-2490-11-19