Study design flowchart. In the first study, sixty knees of the 30 rabbits were randomly divided into 4 groups of 15 knees each in order to clarify the effect of gel implant position (depth from the articular surface) in the defect on quality of the regenerated cartilage. The second study was conducted using 8 rabbits to evaluate gene expression of type-2 collagen, aggrecan, and Sox9 in the tissue regenerated in the defect using real-time PCR analysis.
Matsuda et al. BMC Musculoskeletal Disorders 2013 14:50 doi:10.1186/1471-2474-14-50