Table 1

Antibodies used for the immunocytochemical and immunofluorescence analyses
PRODUCT NAME PRODUCT CODE ANTIGEN DILUTION IMMUNOCYTO CHEMISTRY/IMMUNOFLUORESCENCE DILUTION SECONDARY ANTIBODY COMPANY
Mouse monoclonal to desmin M0760 Desmin 1:100 1:1000 Anti-mouse Dylight 549 DakoCytomation
Mouse monoclonal [F5D] to myogenin Ab1835 Myogenin 1:50 Abcam plc
Mouse monoclonal to troponin T, fast NCL-TROPT TNNT 1:20 NovoCastra™Lyophilized
Mouse monoclonal to titin 3010-S Titin 1:50 BioCytex
Mouse monoclonal to sarcomeric actin M0874 Alpha-skeletal actin 1:10 1:1000 Anti-mouse Dylight 549 DakoCytomation
Mouse monoclonal cardiac actin M622709 Alpha-cardiac actin 1:20 1:1000 Anti-mouse Dylight 549 Nordic Biolabs Bioreagents
Mouse monoclonal to myosin heavy chain, (developmental) NCL-MHCd MyHC-embryonic 1:10 1:1000 Anti-mouse Dylight 549 NovoCastra™Lyophilized
Mouse monoclonal to myosin heavy chain, (neonatal) NCL-MHCn MyHC-neonatal 1:10 1:1000 Anti-mouse Dylight 549 NovoCastra™Lyophilized
Mouse monoclonal to myosin heavy chain, (slow) NCL-MHCs MyHC-slow 1:250 NovoCastra™Lyophilized
Mouse monoclonal to myosin heavy chain, (fast) NCL-MHCf MyHC-fast 1:120 NovoCastra™Lyophilized
Mouse monoclonal to myosin heavy chain, (fast IIa and slow) N2.261 MyHC-fast IIa+slow 1:120 1:1000 Anti-mouse Dylight 549 Santa Cruz Biotechnology
Mouse monoclonal to sarcomeric tropomyosin T9283 TPM 1:100 Sigma-Aldrich
Rabbit polyclonal to beta tropomyosin ARP48224T100 TPM2 1:120 Aviva Systems Biology
Rabbit polyclonal to myosin binding protein C, (slow) HPA021004 MYBPC1-slow 1:50 Sigma-Aldrich
Rabbit polyclonal to myosin binding protein C, (fast) SAB2101539 MYBPC2-fast 1:100 Sigma-Aldrich
Mouse monoclonal [12F10] to skeletal muscle troponin I, (slow) Ab8293 TNNI-slow 1:500 Abcam plc
Mouse monoclonal [2F12A11] to skeletal muscle troponin I, (fast) Ab119943 TNNI- fast 1:200 Abcam plc
Mouse monoclonal [284(19C7)] to cardiac troponin I Ab19615 TNNI- cardiac 1:500 Abcam plc
Mouse monoclonal T12 to Z-disk titin Z-disk 1:20 Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 Fürst et al., 1988
Mouse monoclonal T3 to A/I junction titin A/I junction 1:5 Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 Fürst et al., 1988
Mouse monoclonal T30 to A-band titin A-band 1:5 Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 Fürst et al., 1989
Mouse monoclonal T51 to M-band titin M-band 1:5 Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 Obermann et al., 1996

Abdul-Hussein et al.

Abdul-Hussein et al. BMC Musculoskeletal Disorders 2012 13:262   doi:10.1186/1471-2474-13-262

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