RT-PCR analysis of myogenic regulatory factors (MRFs) and a panel of striated muscle sarcomeric genes in myoblasts and cells differentiated for 6 days. RNA isolated from proliferating mononucleated myoblasts (A, C, E and G) and cultures after 6 days of differentiation (B, D, F and H) was analyzed by RT-PCR and the products were separated on agarose gels. The expression of MRF genes (MYOD1, MYF5 and MYOG), TM isoforms, ACTA1, ACTC1, different MyHC isoforms, DES, TTN and MyBPC isoforms, and troponin I and T isoforms was readily detectable in myoblasts and differentiated cells.
Abdul-Hussein et al. BMC Musculoskeletal Disorders 2012 13:262 doi:10.1186/1471-2474-13-262