Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis
1 Department of Oral Medicine, Taipei Medical University, Taipei, Taiwan
2 Department of Radiology, Taipei Medical University Hospital, Taipei, Taiwan
3 Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan
4 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan
5 Graduate Institute of Biomedical Materials and Engineering Taipei Medical University, Taipei, Taiwan
6 Department of Food and Nutrition, Providence University, Taichung, Taiwan
7 Department of Surgery, School of Medicine, Taipei Medical University-Shuang Ho Hospital, Taipei, Taiwan
8 Department of Physical Medicine and Rehabilitation, Wan Fang Hospital, Taipei Medical University, Taiwan
BMC Musculoskeletal Disorders 2011, 12:227 doi:10.1186/1471-2474-12-227Published: 8 October 2011
Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis.
Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples.
NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group.
iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.