Open Access Research article

Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis

Chiming Lee12, Meileng Cheong3, Wentien Hsiao12, Henyu Liu4, Chingyu Tsai5, Mingfu Wang6, Chihhsiung Wu7, Kwanghwa Chang8, Gowlin Lam5 and Winping Deng5*

Author Affiliations

1 Department of Oral Medicine, Taipei Medical University, Taipei, Taiwan

2 Department of Radiology, Taipei Medical University Hospital, Taipei, Taiwan

3 Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan

4 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan

5 Graduate Institute of Biomedical Materials and Engineering Taipei Medical University, Taipei, Taiwan

6 Department of Food and Nutrition, Providence University, Taichung, Taiwan

7 Department of Surgery, School of Medicine, Taipei Medical University-Shuang Ho Hospital, Taipei, Taiwan

8 Department of Physical Medicine and Rehabilitation, Wan Fang Hospital, Taipei Medical University, Taiwan

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BMC Musculoskeletal Disorders 2011, 12:227  doi:10.1186/1471-2474-12-227

Published: 8 October 2011



Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis.


Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples.


NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group.


iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.

osteoporosis; Quantum Persistent Reflection; mouse fibroblasts; senescence-accelerated mice; bone mass density