Endpoint PCR detection of GR exon 1 mRNA transcript variants in the OFC. A) Strong amplification of the GR-1B, GR-1C and GR-1H variants was evident in OFC cDNA and in universal human cDNA. B) Weaker amplification of the GR-1F variant was observed in the OFC, while strong GR-1F amplification was seen in universal human cDNA. C) No amplification of GR-1E was seen in OFC cDNA, while weak amplification was seen in universal human cDNA, D) No amplification of GR-1A or GR-1D in OFC cDNA or universal human cDNA was detected. Boxes indicate expected amplicon sizes. Abbreviations: Univ- universal human cDNA, bp- base pairs.
Sinclair et al. BMC Psychiatry 2012 12:84 doi:10.1186/1471-244X-12-84