Detection of BDV circulating immunocomplexes (CIC) in serum. a) The antibody against mice IgG (against its Fc fragment) is bound at the surface of the microtitrate plate; b) Mice monoclonal antibody against BDV antigens p40 a p24 is added; c) Patient serum is added (in which we are detecting CIC, ie. the complex of BDV antigen and human IgG); d) Goat antihuman IgG (against its Fc fragmen) conjugated with enzyme (ALP - alcaline phosphatase) is added; e) Substrate of ALP p-nitrophenylphosphate (pNPP) is added; pNPP is hydrolysed by ALP and released p-nitrophenol (pNP) has intensive yellow colour, which is detected by spectrophotometry using microtitrate plate reader (wave length 405 nm).
Rackova et al. BMC Psychiatry 2010 10:70 doi:10.1186/1471-244X-10-70