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Open Access Research article

Prospective assessment of the gastroesophageal microbiome in VLBW neonates

Vladana Milisavljevic12*, Meena Garg2, Ivan Vuletic3, Jeff F Miller3, Lauren Kim2, Tina D Cunningham2 and Imke Schröder3

Author Affiliations

1 Department of Pediatrics, Cedars-Sinai Medical Center, 8700 Beverly Boulevard, NT, Suite 4311, Los Angeles, CA, 90048, USA

2 Department of Pediatrics, Neonatal Research Center, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA

3 Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA

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BMC Pediatrics 2013, 13:49  doi:10.1186/1471-2431-13-49

Published: 5 April 2013

Abstract

Background

The distal GI microbiota of hospitalized preterm neonates has been established to be unique from that of healthy full-term infants; the proximal GI, more specifically gastroesophageal colonization has not been systematically addressed. We prospectively evaluated early colonization of gastroesophageal portion of the GI tract of VLBW infants.

Methods

This study involved 12 infants admitted to a level III NICU with gestational age (GA) 27 +/− 0.5 weeks and birth weight 1105 +/− 77 grams. The gastroesophageal microbial flora was evaluated using 16S rDNA analysis of aspirates collected in a sterile manner during the first 28 days of life.

Results

Bacteria were detected in 9 of the 12 neonates. Ureaplasma was the dominant species in the first week of life, however, staphylococci were the predominant bacteria overall. By the fourth week, Gram (−) bacteria increased in abundance to account for 50% of the total organisms. Firmicutes were present in the majority of the neonates and persisted throughout the 4 weeks comprising nearly half of the sequenced clones. Noticeably, only two distinct species of Staphylococcus epidermidis were found, suggesting acquisition from the environment.

Conclusions

In our neonates, the esophagus and stomach environment changed from being relatively sterile at birth to becoming colonized by a phylogenetically diverse microbiota of low individual complexity. By the fourth week, we found predominance of Firmicutes and Proteobacteria. Bacteria from both phyla (CONS and Gram (−) organisms) are strongly implicated as causes of hospital-acquired infections (HAI). Evaluation of the measures preventing colonization with potentially pathogenic and pathogenic microorganisms from the hospital environment may be warranted and may suggest novel approaches to improving quality in neonatal care.

Keywords:
Colonization; GI microbiome; Neonates; 16S rDNA